does rna polymerase move in a set direction along a strand of dna during transcription? a yes, the rna polymerase moves in a direction that reads the bases of the dna sequence from the 5' end toward the 3' end. b yes, the rna polymerase moves in a direction that reads the bases of the dna sequence from the 3' end toward the 5' end. c no; the rna polymerase can move in either direction along the dna strand because the same sequence of bases in the rna will be produced regardless of direction.

In the 5' to 3' direction, RNA polymerase creates an RNA transcript that is complementary to the DNA template strand. The DNA double helix is opened as it advances down the template strand in the 3' to 5' direction. The DNA helix is unwound right before the active site for polymerization by the RNA polymerase, exposing a fresh area of the template strand for complementary base-pairing. During strand elongation, RNA polymerase starts traveling from 3' to 5' down the DNA template strand while connecting complementary nucleotides. This results in the formation of a new strand of mRNA that is structured in the 5' to 3' direction thanks to complementary base pairing.

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