3. each round of pcr doubles the number of dna molecules present in the reaction. how many molecules can be generated from one of your dna template molecules after 30 cycles?

Answer :

The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 10 cycles you have 1024 copies, after 30 cycles you have about one million copies.

With the aid of the PCR technique, a specific DNA area (known as the target region) that is sandwiched between two sections of known DNA sequence, known as the primers, is amplified exponentially in vitro, yielding a significant amount of DNA (this means micrograms of DNA).

The majority of living things duplicate their DNA in the same way: Using a single-stranded template, DNA polymerases create a complementary strand of DNA in the 5' to 3' orientation. This procedure is mimicked by the PCR, however it is carried out in a test tube and uses two primers, each of which is complementary to the opposing strands of the DNA area. Three temperature-dependent processes are performed in cycles during the PCR amplification:

*1*denaturation\s*2*annealing\s*3*elongation

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