Answer :
The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 10 cycles you have 1024 copies, after 30 cycles you have about one million copies.
With the aid of the PCR technique, a specific DNA area (known as the target region) that is sandwiched between two sections of known DNA sequence, known as the primers, is amplified exponentially in vitro, yielding a significant amount of DNA (this means micrograms of DNA).
The majority of living things duplicate their DNA in the same way: Using a single-stranded template, DNA polymerases create a complementary strand of DNA in the 5' to 3' orientation. This procedure is mimicked by the PCR, however it is carried out in a test tube and uses two primers, each of which is complementary to the opposing strands of the DNA area. Three temperature-dependent processes are performed in cycles during the PCR amplification:
*1*denaturation\s*2*annealing\s*3*elongation
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