A student in high school is performing a science fair project under the guidance
of a University genetics professor. The student wishes to have bacteria make
the human hormone Oxytocin. Explain why the professor told the student that they would need to start with a secondary transcript when making the recombinant plasmid?


Answer :

The professor needs a secondary transcript when making the recombinant plasmid because it is used as a marker. A molecular marker can be used to reveal the expression of another gene.

Plasmids and molecular markers

A plasmid is a circular fragment of a DNA molecule, which is not contained in the genome of an organism (single chromosome in bacteria).

A molecular marker is any sequence that is used to determine the presence/expression of another sequence in a given genetic material.

In this case, the marker can be used to reveal the presence of the oxytocin protein-coding gene within the plasmid.

Learn more about molecular markers here:

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