What is the concentration (in m) of a sample of the unknown dye with an absorbance of 0. 25 at 542 nm?.

Answer :

The concentration of the dye at the absorbance of 0.25 = 1.76x10⁻⁵M

The regression equation says

y = 15200x - 0.018

with y = 0.25

y is the absorbance and x is the concentration

We have to put in the value of y to get the concentration x

y = 15200x - 0.018

0.25 = 15200x - 0.018

0.25+0.018 =  15200x

0.268 = 15200x

x = 0.268/15200

= 1.76x10⁻⁵M

The concentration of the dye at the absorbance of 0.25 = 1.76x10⁻⁵M

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