The benefit of measuring the initial rate of a reaction, Vo, is that early in the course of the conversion of substrate (s) to products,
i. All the enzyme will still be present.
ii. Vo will be closest to Vmax.
iii. The rate of product formation is linear and the concentration of substrate will not have been lowered significantly.
iv. Changes in substrate concentration have the least effect on Vo.
v. The enzyme will be saturated with substrate.
Which of the following statements about a plot of Vo versus [S] for an enzyme that follows Michaelis-Menten kinetics is true?
i. Km is the [S] at which Vo has become equal to Vmax-
ii. The y-axis has units of concentration.
iii. The plot is a curve that becomes steeper as [S] becomes larger.
iv. The plot gives a straight line.
v. At very high [S], the Vo versus [S] curve approaches a horizontal line that, when extrapolated back, intersects the y-axis at Vmax